How many samples can I fix at a time?
Between 1-96 samples can be fixed in parallel with EvercodeTM Cell Fixation v3 and Evercode Nuclei Fixation v3 kits for inputs of 100,000 to 4M1 cells/nuclei or Evercode Low Input Cell Fixation v3 and Evercode Low Input Nuclei Fixation v3 for inputs from 10,000 to 100,000 cells/nuclei. Depending on the number of samples being processed, we recommend different workflows and kits. See the tables below for detailed recommendations.
Note: In order to maximize the cell/nuclei quality, high-throughput fixation kits are only recommended for projects where 48-96 samples can be prepared at the same time.
- Cell and Nuclei Fixation kits
Input (cells/nuclei per sample)
|# samples to fix at the same time
|Format
|Strainer
|Cells
|Nuclei
PN
|User Guide
|PN
|User Guide
Standard3
100,000 to 1M
|1-12
|1.5 mL tube
or Plate-
based3
|Not included
|ECFC3300
|Evercode Cell Fixation v3 User Manual
|ECFN3300
|Mid-
Throughput
12-482
|Plate-
based
|30µM
|ECFC3301
|Evercode Cell Fixation v3 Mid-Throughput Plate Workflow
|ECFN3301
|70µM
|ECFC3303
100µM
|ECFC3305
High-
Throughput
48-96
|Plate-
based
|30µM
|ECFC3501
|High-Throughput Evercode Cell Fixation v3 User Manual
|ECFN3501
|70µM
|ECFC3503
100µM
|ECFC3505
Low
10,000 to 100,000
|1-12
|0.2 mL tube
|30µM
|ECLC3301
|Evercode Low Input Cell and Nuclei Fixation v3 User Manual
|ECLN3301
|70µM
|ECLC3303
100µM
|ECLC3305
Mid-
Throughput
12-482
|Plate-
based
|30µM
|ECLC3301
|ECLN3301
70µM
|ECLC3303
100µM
|ECLC3305
High-
Throughput
48-96
|Plate-
based
|30µM
|ECLC3501
|ECLN3501
70µM
|ECLC3503
100µM
|ECLC3505
High
1M to
4M
|1-3
|15 mL tube
|Not included
|ECFC3300
|Appendix B of Evercode Cell Fixation v3 User Manual
|ECFN3300
|Appendix B of Evercode Nuclei Fixation v3 User Manual
1When using the High Input workflow, up to 3 samples can be fixed with one kit (PN ECFC3300 or ECFN3300).
2Mid-Throughput workflow requires multiple kits. Each part number ECFC3301, ECFC3303, ECFC3305, ECLC3301, ECLC3303, ECLC3305, ECFN3301, and ECLN3301 provides reagents for fixing up to 12 samples.
3Parse Evercode™ Cell and Nuclei Fixation workflows can be semi-automated using the INTEGRA ASSIST PLUS platform. For more information check the following link: Evercode Cell and Nuclei Fixation v3 and INTEGRA ASSIST PLUS Scripts