General Questions

FAQ

• What is the range of cells and nuclei that can be input into fixation?
• Will I pickup ambient RNA in my data?
• What required equipment do I need to run a kit?
• What is a sublibrary?
• Do I need to purchase a custom instrument to run a Parse kit?
• What are unique dual indices (UDIs)?
• What instrument should I use for sequencing?

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Experimental Design and Workflow

• Can I use the same sample in multiple barcoding runs?
• Can I preserve my samples before doing my single cell experiment?
• Can I sequence a subset of cells or nuclei from a single experiment?
• Do I have to use the Qubit to measure DNA concentrations?
• Do Parse kits use standard Illumina primers?