What vectors are CRISPR Detect compatible with?
CRISPR Detect is compatible with CROP-seq methods that generate a polyadenylated transcript expressed downstream of a human U6 promoter. The table below includes some examples, but it is not exhaustive. Please also see the CRISPR Detect Vector Compatibility Guide for more information.
- Vector: CROPseq-Guide-Puro; Selection: Puromycin; Addgene Number: 86708; Pubmed ID: 28099430
- Vector: CROPseq-puro-v2; Selection: Puromycin; Other Features: Modified sgRNA scaffold for increased Cas9 efficiency; Addgene Number: 127458; Pubmed ID: 31626775
- Vector: CROPseq-Guide-Zeo; Selection: Zeocin; Addgene Number: 127173; Pubmed ID: 31626775
- Vector: CROP-seq-opti; Selection: Puromycin; Other Features: CRISPRi optimized backbone; Addgene Number: 106280; Pubmed ID: 29457792
- Vector: CROP-sgRNA-MS2; Selection: Puromycin or mCherry; Other Features: sgRNA scaffold sequence contains two MS2; Addgene Number: 153457; Pubmed ID: 32634384
- Vector: CROPseq-Guide-EFS-SpCas9-P2A-EGFP; Selection: EGFP; Other Features: EF-1α replaced with EFS promoter; Addgene Number: 99248
- Vector: EFS-EGFP-P2A-Neo-WPRE-U6-AsCas12aDR-AsCas12aDR (pRG212); Selection: Neomycin or EGFP; Other Features: Cas12a scaffold, enabling dual guide screening; Addgene Number: 149722; Pubmed ID: 32661245
- Vector: Crop-Seq-BFP-WPRE-TS-hU6-BsmbI; Selection: BFP; Other Features: Lineage tracing vector system; Addgene Number: 137993; Pubmed ID: 35094325