How are Custom Gene Panels designed?

We developed an approach to design panels compatible with Evercode Whole Transcriptome libraries. From a gene list, the MANE transcript of the gene is chosen, and if a MANE transcript is not available, the ENSEMBL canonical transcript is used instead. The full CDS and the 3’ UTR of this transcript are used to design end-to-end non-overlapping 120 base pair probes (1x tiling).

If genomic coordinates or FASTA files are supplied, these regions are used directly to design end-to-end non-overlapping 120 base pair probes (1x tiling).