Parse Biosciences - Scalable Single Cell Sequencing

2023 Alzheimer’s and Brain Awareness Month

Thank you for joining us in celebrating the spirit of discovery during June's Alzheimer’s and Brain Awareness Month!

We have curated a selection of neuro-focused webinars, datasets, tech notes, and publications. 

Webinars

Zac Moore, PhD
Uncovering Glioblastoma Heterogeneity in Solid Tumor and Tumor-derived in vitro Models
Zac Moore, PhD
WEHI (Walter and Eliza Hall Institute)

▶️ Watch Recorded Webinar
Vivek Swarup, PhD
Single Cell RNA-seq Sheds Light on the Role of Microglia in Alzheimer’s Disease
Vivek Swarup, PhD
Institute for Memory Impairments and Neurological Disorders
Department of Neurobiology and Behavior
University of California, Irvine

▶️ Watch Recorded Webinar
Narges Rezaie
Identifying Distinct Cellular Programs from Single Cell Datasets Using Topyfic
Narges Rezaie
UC Irvine

▶️ Watch Recorded Webinar
Federico Gaiti, PhD
Spatial Heterogeneity of Transcriptomic Variability in Glioblastoma
Federico Gaiti, PhD
Princess Margaret Cancer Centre

▶️ Watch Recorded Webinar

Resources

Uncovering Spatial Intra-Tumoral Heterogeneity of Malignant and Immune Cells in Glioblastoma
Our customer used the Parse Evercode WT Mini kit to profile nuclei from 4 tumors collected from 4 patients, providing insights into the mechanisms behind the diverse phenotypes observed in GBM and aiding the development of targeted therapies.
Tech Note

A head-to-head evaluation of single cell RNA-seq technologies between droplet-based microfluidics (10x Genomics Chromium™ Next GEM Single Cell 3’ Kit v3.1) and combinatorial barcoding (Parse Biosciences Evercode WT v2) using brain tissue.

Uncovering Glioblastoma Heterogeneity in Solid Tumor and Tumor-derived in vitro Models
This study aimed to understand the heterogeneity of glioblastoma, a prevalent form of brain cancer, by analyzing tumor samples and in vitro models. Using the Parse Evercode v2 assay, the researchers profiled whole cells processed from a patient-derived neurosphere line, alongside nuclei isolated from a piece of flash-frozen glioblastoma tissue. In-depth understanding of neurosphere diversity can inform glioblastoma drug development.
Performance of Evercode™ WT in Adult Mouse Brain Cells
Adult brain cells have relatively larger sizes, which are incompatible with microfluidic approaches. Evercode technology overcomes the limitation of cell size by enabling the collection of cytoplasmic transcripts even from large cells. Fresh brain tissue was collected from an adult C57/BL6 mouse. After mechanical tissue dissociation and percoll clean-up, the single cell suspension was fixed with Evercode Cell Fixation v2 and whole transcriptome libraries were generated with Evercode WT v2.
Single Cell RNA-Seq Sheds Light on the Role of Microglia in Alzheimer’s Disease
The absence of microglia in Alzheimer's mice leads to severe pathology, but injecting adult microglia prevents these changes, highlighting their protective role. The data obtained using the Parse WT kit on brain tissue nuclei revealed diverse neuronal, glial, and vascular cell types, including region-specific excitatory and inhibitory neurons, astrocytes, oligodendrocytes, and an immune cell population with microglia signatures.


Publications & Posters

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