Single cell pooled CRISPR screens enable analysis of complex phenotypes by linking individual gene perturbations with gene expression profiles. This approach is used for target validation in drug discovery, to understand differences in treatment responses, and map pathways involved in cell differentiation. Wider adoption of this approach is constrained by the feasibility of processing sufficient cells for libraries containing more than a few hundred guide RNAs (sgRNAs).
In this seminar, we will demonstrate how CRISPR Detect brings the scalability of the Parse Biosciences Evercode technology to single cell CRISPR screens, making genome-wide studies practical.
